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Image Search Results
Journal: Stem Cells Translational Medicine
Article Title: Whole-genome CRISPR screening identifies genetic modifiers of stem cell–derived islet transplantation
doi: 10.1093/stcltm/szag012
Figure Lengend Snippet: Transplantation of CRISPR-activation of FCAMR , GSTM3 , and SIX3 . (A-C) Blood glucose of 10 million genetically engineered SC-islet cells transplanted into the subcutaneous space ( n = 5). (A) FCAMR overexpression and control SC-islet cells, (B) GSTM3 overexpression and control SC-islet cells, and (C) SIX3 overexpression and control SC-islet cells. * P < .05 by 2-way paired t -test comparing control and overexpression. (D - F) C-peptide measurements from week 5 and 9 after a 30-min 3 g/kg intraperitoneal glucose injection ( n = 5). (D) FCAMR overexpression and control SC-islet cells, (E) GSTM3 overexpression and control SC-islet cells, and (F) SIX3 overexpression and control SC-islet cells. NSG male mice were used for this experiment. Source Data can be found in the Source Data file.
Article Snippet: C-peptide was measured with the
Techniques: Transplantation Assay, CRISPR, Activation Assay, Over Expression, Control, Injection
Journal: Stem Cells Translational Medicine
Article Title: Whole-genome CRISPR screening identifies genetic modifiers of stem cell–derived islet transplantation
doi: 10.1093/stcltm/szag012
Figure Lengend Snippet: Kidney and muscle transplantations of SC-islet overexpressing FCAMR. (A, E) Schematic of SC-islet cells transplanted into two groups of mice ( N = 6–8): (A) 5 million into the kidney capsule. (E) 10 million intramuscularly. (B, F) Blood glucose measurements before inducing diabetes, 5-day injections of streptozotocin (STZ), and post-STZ. ** P < .01 by way of 2-way unpaired t -test comparing control (CTRL) and FCAMR overexpressing ( FCAMR OE) SC-islets in (B) kidney capsule or (F) intramuscular transplants. (C, G) C-peptide measurements after a 30-min 3 g/kg intraperitoneal glucose injection of SC-islets transplanted: (C) into the kidney capsule or (G) intramuscularly. (D, H) Bar graphs of weights of mice before transplantation (week 0), after STZ injection (week 6), and before nephrectomy (week 11) in (D) kidney transplant or (H) intramuscular transplanted mice. *** P < .001, ** P < .01, and * P < .05 by way of 2-way unpaired or paired t -test. (I) Immunohistochemistry of kidney grafts stained with human C-peptide (green), CHGA (red), and DAPI (blue) on the left, GCG (green), SST (red), and DAPI (blue) in the middle, and SYP (green), Ki67 (red), and DAPI (blue) on the right. Scale bar: 200 µM. Source Data can be found in the Source Data file.
Article Snippet: C-peptide was measured with the
Techniques: Control, Injection, Transplantation Assay, Immunohistochemistry, Staining